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KMID : 0380420010250040277
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Journal of Prventive Veterinary Medicine
2001 Volume.25 No. 4 p.277 ~ p.287
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Antimicrobial Activity of Lactobacillus reuteri against Bacillus anthracis Sterne 34 F2
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Bae Won-Gi
Kim Ji-Youn
Lim Ji-Yeun
Noh Kyoung-Min
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Abstract
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Antimicrobial activities of three different Lactobacillus strains (Lactobacillus reuteri, L. bulgaricus, and L. casei) against Bacillus anthracis were determined on the Mueller Hinton Agar containing each culture supernatant obtained from 3 different growth conditions (MRS without glycerol, MRS with 0.5 M glycerol and 0.25 M glycerol solution).Though antimicrobial activity of L. reuteri in the first two conditions was not better than the others´, the activity was significantly higher than those of others in 0.25 M glycerol solution. This prominent effect might be attributable to reuterin, produced by L. reuteri using glycerol. We could detect the presence of reuterin in the supernatant of 0.25 M glycerol solution with 500 MHz Nuclear Magnetic Resonance (NMR). The result of minimum bactericidal concentration (dilution fold) against spore of B. anthracis has revealed that reuterin had strong effect against anthrax spores. To examine any changes of antimicrobial activities of the probiotics, the probiotics were treated with different pH concentration, pepsin or trypsin digestion. The study has revealed that antimicrobial activities of L. bulgaricus and L. casei were significantly decreased, but reuterin activity was not entirely affected by any of these treatments.
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